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Plasmid-mediated AmpC β-lactamase gene PCR

Antibiotic Reference Laboratory

Demonstration of the presence of plasmid-mediated AmpC β-lactamase genes by multiplex PCR.

The test is appropriate for cefoxitin-resistant E. coli, Klebsiella spp and Salmonella spp. Prior to performing the PCR, ESR will screen the isolate for AmpC β-lactamase using a boronic acid double-disc synergy test.



Health Science

Lab name

Antibiotic Reference Laboratory

Lab location

Kenepuru Science Centre, Porirua

Test name

Plasmid-mediated AmpC β-lactamase gene PCR


Escherichia coli, Klebsiella spp and Salmonella spp

Requested for surveillance


Notice required


Routine turnaround time

10 working days for reference specimens

Sample requirements

Pure culture

Media requirements

A slope of any medium that supports the growth of the organism

Pre-transport requirements

Ambient temperature

Transport requirements

Ambient temperature

Unacceptable sample types

Clinical specimens will not be accepted.

How results reported

The results will state if a plasmid-mediated AmpC β-lactamase was detected, and if so, what group was detected

Referral link