Demonstration of the presence of plasmid-mediated AmpC β-lactamase genes by multiplex PCR.
The test is appropriate for cefoxitin-resistant E. coli, Klebsiella spp and Salmonella spp. Prior to performing the PCR, ESR will screen the isolate for AmpC β-lactamase using a boronic acid double-disc synergy test.
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Area |
Health Science |
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Lab name |
Antibiotic Reference Laboratory |
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Lab location |
Kenepuru Science Centre, Porirua |
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Test name |
Plasmid-mediated AmpC β-lactamase gene PCR |
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Organisms |
Escherichia coli, Klebsiella spp and Salmonella spp |
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Requested for surveillance |
no |
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Notice required |
no |
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Routine turnaround time |
10 working days for reference specimens |
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Sample requirements |
Pure culture |
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Media requirements |
A slope of any medium that supports the growth of the organism |
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Pre-transport requirements |
Ambient temperature |
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Transport requirements |
Ambient temperature |
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Unacceptable sample types |
Clinical specimens will not be accepted. |
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How results reported |
The results will state if a plasmid-mediated AmpC β-lactamase was detected, and if so, what group was detected |
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Referral link |