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Plasmid-mediated AmpC β-lactamase gene PCR

Antibiotic Reference Laboratory

Demonstration of the presence of plasmid-mediated AmpC β-lactamase genes by multiplex PCR.

The test is appropriate for cefoxitin-resistant E. coli, Klebsiella spp and Salmonella spp. Prior to performing the PCR, ESR will screen the isolate for AmpC β-lactamase using a boronic acid double-disc synergy test.

 

Area

Health Science

Lab name

Antibiotic Reference Laboratory

Lab location

Kenepuru Science Centre, Porirua

Test name

Plasmid-mediated AmpC β-lactamase gene PCR

Organisms

Escherichia coli, Klebsiella spp and Salmonella spp

Requested for surveillance

no

Notice required

no

Routine turnaround time

10 working days for reference specimens

Sample requirements

Pure culture

Media requirements

A slope of any medium that supports the growth of the organism

Pre-transport requirements

Ambient temperature

Transport requirements

Ambient temperature

Unacceptable sample types

Clinical specimens will not be accepted.

How results reported

The results will state if a plasmid-mediated AmpC β-lactamase was detected, and if so, what group was detected

Referral link

Form