This website has changed. We hope you can find what you need easily, but items have moved around. If you have trouble finding what you are looking for please let us know.

Contact us

OXA-23-like, OXA-40-like, OXA-58-like and OXA-51-like carbapenemase gene PCR

Antibiotic Reference Laboratory

Demonstration of the presence of an OXA carbapenemase gene by PCR.

Carbapenemase-mediated carbapenem resistance in Acinetobacter is most often due to the production of an OXA-23-like, OXA-40-like or OXA-58-like carbapenemase. In addition, carbapenem resistance in Acinetobacter may be due to overexpression of an OXA-51-like carbapenemase. OXA-51-like carbapenemases are intrinsic in A. baumannii and normally expressed at low levels but they can be overexpressed following the insertion of an ISAba1 sequence element upstream of the OXA-51 gene.

To check for the presence of OXA-23-like, OXA-40-like, OXA-58-like and OXA-51-like genes, a multiplex PCR is used and contains primers for each of the four groups of OXA-type carbapenemases. Any Acinetobacter isolates that are carbapenem resistant, but only positive for OXA-51-like genes in the multiplex PCR, are tested by PCR for the ISAba1 insertion sequence.

This test is appropriate for carbapenem-resistant Acinetobacter or Pseudomonas isolates.

 

Area

Health Science

Lab name

Antibiotic Reference Laboratory

Lab location

Kenepuru Science Centre, Porirua

Test name

OXA-23-like, OXA-40-like, OXA-58-like and OXA-51-like carbapenemase gene PCR

Organisms

Acinetobacter and Pseudomonas

Requested for surveillance

no

Notice required

no

Routine turnaround time

6 working days for reference specimens

Sample requirements

Pure culture

Media requirements

A slope of any medium that supports the growth of the organism

Pre-transport requirements

Ambient temperature

Transport requirements

Ambient temperature

Unacceptable sample types

Clinical specimens will not be accepted.

How results reported

The results will state whether OXA-23-like, OXA-40-like, OXA-58-like or OXA-51-like genes or the IS Aba1 insertion sequence were detected

Referral link

Form