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IMP, VIM, NDM, SIM, GES and IMI-carbapenemase gene PCR

Antibiotic Reference Laboratory

Demonstration of the presence of IMP, VIM or NDM metallo-β-lactamase genes by PCR. The test is appropriate for ceftazidime-resistant Enterobacterales, Pseudomonas and Acinetobacter with carbapenem resistance or reduced susceptibility.

Prior to performing the PCR, ESR will screen the isolate for metallo-β-lactamase using the CLSI modified carbapenem inactivation method, and EDTA and MPA/TE inhibitor-based double-disc synergy tests.

 

Area

Health Science

Lab name

Antibiotic Reference Laboratory

Lab location

Kenepuru Science Centre, Porirua

Test name

IMP, VIM, NDM, SIM, GES and IMI-carbapenemase gene PCR

Organisms

Enterobacterales, Pseudomonas and Acinetobacter

Requested for surveillance

yes

Notice required

no

Routine turnaround time

6 working days for reference specimens

Sample requirements

Pure culture

Media requirements

A slope of any medium that supports the growth of the organism

Pre-transport requirements

Ambient temperature

Transport requirements

Ambient temperature

Unacceptable sample types

Clinical specimens will not be accepted.

How results reported

The results will state whether IMP, VIM or NDM genes were detected

Referral link

Form