Demonstration of the presence of IMP, VIM or NDM metallo-β-lactamase genes by PCR. The test is appropriate for ceftazidime-resistant Enterobacterales, Pseudomonas and Acinetobacter with carbapenem resistance or reduced susceptibility.
Prior to performing the PCR, ESR will screen the isolate for metallo-β-lactamase using the CLSI modified carbapenem inactivation method, and EDTA and MPA/TE inhibitor-based double-disc synergy tests.
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Area |
Health Science |
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Lab name |
Antibiotic Reference Laboratory |
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Lab location |
Kenepuru Science Centre, Porirua |
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Test name |
IMP, VIM, NDM, SIM, GES and IMI-carbapenemase gene PCR |
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Organisms |
Enterobacterales, Pseudomonas and Acinetobacter |
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Requested for surveillance |
yes |
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Notice required |
no |
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Routine turnaround time |
6 working days for reference specimens |
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Sample requirements |
Pure culture |
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Media requirements |
A slope of any medium that supports the growth of the organism |
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Pre-transport requirements |
Ambient temperature |
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Transport requirements |
Ambient temperature |
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Unacceptable sample types |
Clinical specimens will not be accepted. |
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How results reported |
The results will state whether IMP, VIM or NDM genes were detected |
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Referral link |