ESBLs belong to three main families of β-lactamases: TEM, SHV and CTX-M. The CTX-M ESBLs are grouped, on the basis of sequence similarity, into five main groups: 1, 2, 8, 9 and 25. To identify a specific ESBL, sets of consensus primers, which amplify all TEM genes, all SHV genes and identify the CTX-M group, are used in separate PCR reactions. Any products of the correct size from the TEM and SHV PCRs are sequenced to identify the specific TEM or SHV β-lactamase. The specific CTX-M type can be identified by sequencing using CTX-M group-specific primers.
Identification of the ESBL type is appropriate for outbreak strains of ESBL-producing Enterobacterales.
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Area |
Health Science |
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Lab name |
Antibiotic Reference Laboratory |
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Lab location |
Kenepuru Science Centre, Porirua |
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Test name |
Extended-spectrum β-lactamase (ESBL) gene PCR and sequencing |
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Organisms |
Enterobacterales |
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Requested for surveillance |
no |
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Notice required |
no |
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Routine turnaround time |
20 working days for reference specimens |
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Sample requirements |
Pure culture |
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Media requirements |
A slope of any medium that supports the growth of the organism |
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Pre-transport requirements |
Ambient temperature |
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Transport requirements |
Ambient temperature |
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Unacceptable sample types |
Clinical specimens will not be accepted. |
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How results reported |
The results will state the ESBL type or CTX-M group |
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Referral link |