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RNA stable isotope probing and high-throughput sequencing to identify active microbial community members in a methane-driven denitrifying biofilm

Abstract

Aerobic methane oxidation coupled to denitrification (AME‐D) is a promising process for removing nitrate from groundwater and yet its microbial mechanism and ecological implications are not fully understood. This study used RNA stable isotope probing (RNA‐SIP) and high‐throughput sequencing to identify the micro‐organisms that are actively involved in aerobic methane oxidation within a denitrifying biofilm.

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