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Laser microdissection methodology in forensic casework

Abstract

Laser microdissection (LMD) is a tool used in forensic laboratories for the analysis of DNA from specifically targeted cells. In our laboratory, LMD analysis is applied to case samples where it has been shown (or it is anticipated) that there are small numbers of target cells present, such as sperm. In this paper, we extend previous LMD technology developed in this laboratory1. A method for improved recovery and visualisation of cells from substrates for downstream laser microdissection and DNA analysis is discussed and the optimal numbers of LMD-recovered sperm and epithelial cells for Low Copy Number (LCN) DNA analysis is determined.

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