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Investigating the potential of body fluid identification using the Oxford Nanopore MinION.

Abstract

As the interest in massively parallel sequencing (MPS) in forensic science continues to grow, the technology available to do such work is rapidly developing. An instrument of particular interest is the Oxford Nanopore MinION, a portable USB sequencer. In addition to shorter library preparation time, the MinION sequencer produces ultra-long reads allowing for sequencing of full-length transcripts. Direct RNA and cDNA kits eliminate the need for PCR. However, PCR-free methods require relatively high amounts of input RNA, which could be problematic with the small, degraded samples common in forensic casework. We have initially assessed the performance and feasibility of the MinION for forensic RNA using salivary gland and tongue RNA.

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