Effects of histological staining on the analysis of human DNA from archived slides

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Effects of histological staining on the analysis of human DNA from archived slides

Abstract

Archived slides of cell smears treated with histological stains for sperm detection are often the only source of DNA available when cold cases are reopened. There have been conflicting reports as to the negative effects of particular histological stains on DNA recovery and quality from human cells, making stain selection an important consideration for forensic laboratories. This study investigates the effect of several staining systems on DNA recovery from histological slide samples stored from 0 to 10 weeks. DNA profiles obtained after analysis of these samples with AmpFlSTR(

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Effects of histological staining on the analysis of human DNA from archived slides

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Effects of histological staining on the analysis of human DNA from archived slides

Abstract

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