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A novel magnetic capture-multiplex PCR assay for the simultaneous detection of three foodborne pathogens

Abstract

Salmonella, Shigella and Escherichia coli O157: H7 are major foodborne pathogens that cause gastrointestinal diseases worldwide. Apart from food contamination, fecal pollution has been consistently associated with the transmission of these pathogens, and their rapid detection in food and stools is of significance for food safety. However, a variety of factors associated with these complex samples can decrease the sensitivity and specificity of molecular‐based methods for detection of these pathogens.

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