Plasmid-mediated AmpC β-lactamase gene PCR
|Lab name||Antibiotic Reference Laboratory|
|Lab location||Kenepuru Science Centre, Porirua|
|Test name||Plasmid-mediated AmpC β-lactamase gene PCR|
|Organisms||Escherichia coli, Klebsiella spp and Salmonella spp|
|Requested for surveillance||no|
|Routine turnaround time||10 working days for reference specimens|
|Culture requirements||Pure culture|
|Media requirements||A slope of any medium that supports the growth of the organism|
|Pre-transport requirements||Ambient temperature|
|Transport requirements||Ambient temperature|
|Unacceptable sample types||Clinical specimens will not be accepted.|
|How results reported||The results will state if a plasmid-mediated AmpC β-lactamase was detected, and if so, what group was detected|
Demonstration of the presence of plasmid-mediated AmpC β-lactamase genes by multiplex PCR.
The test is appropriate for cefoxitin-resistant E. coli, Klebsiella spp and Salmonella spp. Prior to performing the PCR, ESR will screen the isolate for AmpC β-lactamase using a boronic acid double-disc synergy test.