mecA gene PCR
|Lab name||Antibiotic Reference Laboratory|
|Lab location||Kenepuru Science Centre, Porirua|
|Test name||mecA gene PCR|
|Organisms||Staphylococci with borderline oxacillin susceptibility|
|Requested for surveillance||no|
|Routine turnaround time||6 working days for reference specimens|
|Culture requirements||Pure culture|
|Media requirements||A slope of any medium that supports the growth of the organism|
|Pre-transport requirements||Ambient temperature|
|Transport requirements||Ambient temperature|
|Unacceptable sample types||Clinical specimens will not be accepted.|
|How results reported||The results will state whether the mecA gene was detected|
Demonstration of the presence of the mecA gene by PCR.
The test is appropriate for isolates with borderline oxacillin susceptibility to confirm mecA-mediated oxacillin resistance and to differentiate mecA-mediated oxacillin resistance from other mechanisms of oxacillin resistance (for example, hyperproduction of β-lactamase).