KPC carbapenemase gene PCR
|Lab name||Antibiotic Reference Laboratory|
|Lab location||Kenepuru Science Centre, Porirua|
|Test name||KPC carbapenemase gene PCR|
|Requested for surveillance||yes|
|Routine turnaround time||6 working days for reference specimens|
|Culture requirements||Pure culture|
|Media requirements||A slope of any medium that supports the growth of the organism|
|Pre-transport requirements||Ambient temperature|
|Transport requirements||Ambient temperature|
|Unacceptable sample types||Clinical specimens will not be accepted.|
|How results reported||The results will state whether the KPC gene was detected|
Demonstration of the presence of a KPC carbapenemase gene by PCR.
KPC carbapenemases are most commonly identified in Klebsiella pneumoniae, but have also been identified in other Enterobacteriaceae. The KPC β-lactamase confers resistance to all β-lactams, including cephalosporins, monobactams and carbapenems. Several types of KPC carbapenemase have been described.
This test is appropriate for isolates that screen positive for carbapenemase in the CLSI modified carbapenem inactivation method.