IMP, VIM and NDM carbapenemase gene PCR
|Lab name||Antibiotic Reference Laboratory|
|Lab location||Kenepuru Science Centre, Porirua|
|Test name||IMP, VIM and NDM carbapenemase gene PCR|
|Organisms||Enterobacteriaceae, Pseudomonas and Acinetobacter|
|Requested for surveillance||yes|
|Routine turnaround time||6 working days for reference specimens|
|Culture requirements||Pure culture|
|Media requirements||A slope of any medium that supports the growth of the organism|
|Pre-transport requirements||Ambient temperature|
|Transport requirements||Ambient temperature|
|Unacceptable sample types||Clinical specimens will not be accepted.|
|How results reported||The results will state whether IMP, VIM or NDM genes were detected|
Demonstration of the presence of IMP, VIM or NDM metallo-β-lactamase genes by PCR. The test is appropriate for ceftazidime-resistant Enterobacteriaceae, Pseudomonas and Acinetobacter with carbapenem resistance or reduced susceptibility.
Prior to performing the PCR, ESR will screen the isolate for metallo-β-lactamase using the CLSI modified carbapenem inactivation method, and EDTA and MPA/TE inhibitor-based double-disc synergy tests.