Breadcrumbs

Isolation of Legionella from Environmental Sources

Registry Details

Area Water Science
Lab name Environmental Microbiology Laboratory
Lab location Kenepuru Science Centre, Porirua
Test name Isolation of Legionella from Environmental Sources
Organisms Legionella species
Requested for surveillance no
Notice required yes
Routine turnaround time 7 working days
Culture requirements Water or biofilm sample
Pre-transport requirements Samples must be sent immediately by courier so as to arrive within 24 hours of collection. Avoid freezing. Avoid temperature fluctuations.
Transport requirements Send immediately at ambient temperature (10-20°C) with no ice packs in a chilly bin. Send protected from light.
Unacceptable sample types Samples that arrive more than 48 hours after collection. Samples that have been frozen. Samples without biocide inhibitors included. Biofilm sample with no source water included to prevent sample drying out in transit. Swab samples that contain no source water.
How results reported Positive or negative for the detection of Legionella bacteria.
Referral link Form

Details

Full details for the collection of environmental samples to be examined for the presence of Legionella bacteria when investigating the source of infection in cases of legionellosis can be found in these guidelines 'Environmental Sampling for Legionella Bacteria' [PDF, 468 KB] or contact lab staff via KSC.Legionella@esr.cri.nz or phone 04 914 0755.

Sample TypeRequired sample volume
Potable water (domestic outlet, i.e. shower, tap) A minimum of 1 L collected in a sterile leak-proof plastic container with 200 mg/L of sodium thiosulphate
Potable water source supply A minimum of 10 L collected in a sterile leak-proof plastic container with 200 mg/L of sodium thiosulphate.
Cooling tower water 250 mL collected in a sterile leak-proof container with 200 mg/L of sodium thiosulphate.
Ornamental water source A minimum of 1 L collected in a sterile leak-proof plastic container with 200 mg/L of sodium thiosulphate.
Spa pool or recreational water A minimum of 1 L collected in a sterile leak-proof plastic container with 200 mg/L of sodium thiosulphate.
Biofilm sample Cotton swab in sealed container with 1-2 mL the source water.
Compost /potting mix/seed raising mix/soil 100 grams in clean and unused food grade zip-lock bag.
Air sample Contact laboratory KSC.Legionella@esr.cri.nz