Legionella detection by PCR
|Lab name||Legionella Reference Laboratory|
|Lab location||Kenepuru Science Centre, Porirua|
|Test name||Legionella detection by PCR|
|Requested for surveillance||yes|
|Routine turnaround time||5-10 working days|
|Culture requirements||Lower respiratory tract samples, including bronchial washing, expectorated sputum, induced sputum, trans-tracheal aspirate, bronchoalveolar lavage fluid, lung biopsy or post-mortem tissue|
|Pre-transport requirements||All samples should be sent to the laboratory at 2-8°C within 24hrs of collection. For longer storage or delays in transportation, store frozen at -20°C and send samples frozen. Avoid freeze-thawing cycles.|
|Transport requirements||Send chilled (2-8°C) within 24 hours|
|Unacceptable sample types||Positive or negative for the detection of Legionella|
|How results reported||Positive or negative for the detection of Legionella|
The Legionella PCR test is carried out weekly with a minimum turnaround time of 5 working days. The Legionella PCR test uses primers specific to the 16S rRNA gene of Legionella.
DNA sequencing confirmation of positive tests will identify the Legionella agent to the species level.
A negative result reflects no Legionella detected in the specimen, but does not eliminate Legionella as the causative agent. A positive result proves the presence of Legionella in the sample.
Information sheet on Legionella and Legionellosis [PDF, 137 KB]