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Plasmid-mediated AmpC β-lactamase gene PCR

Registry Details

Area Health Science
Lab name Antibiotic Reference Laboratory
Lab location Kenepuru Science Centre, Porirua
Test name Plasmid-mediated AmpC β-lactamase gene PCR
Organisms Escherichia coli, Klebsiella spp and Salmonella spp
Requested for surveillance no
Notice required no
Routine turnaround time 10 working days for reference specimens
Culture requirements Pure culture
Media requirements A slope of any medium that supports the growth of the organism
Pre-transport requirements Ambient temperature
Transport requirements Ambient temperature
Unacceptable sample types Clinical specimens will not be accepted.
How results reported The results will state if a plasmid-mediated AmpC β-lactamase was detected, and if so, what group was detected
Referral link Form

Details

Demonstration of the presence of plasmid-mediated AmpC β-lactamase genes by multiplex PCR.

 

The test is appropriate for cefoxitin-resistant E. coli, Klebsiella spp and Salmonella spp. Prior to performing the PCR, ESR will screen the isolate for AmpC β-lactamase using a boronic acid double-disc synergy test.