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OXA-23-like, OXA-40-like, OXA-58-like and OXA-51-like carbapenemase gene PCR

Registry Details

Area Health Science
Lab name Antibiotic Reference Laboratory
Lab location Kenepuru Science Centre, Porirua
Test name OXA-23-like, OXA-40-like, OXA-58-like and OXA-51-like carbapenemase gene PCR
Organisms Acinetobacter and Pseudomonas
Requested for surveillance no
Notice required no
Routine turnaround time 6 working days for reference specimens
Culture requirements Pure culture
Media requirements A slope of any medium that supports the growth of the organism
Pre-transport requirements Ambient temperature
Transport requirements Ambient temperature
Unacceptable sample types Clinical specimens will not be accepted.
How results reported The results will state whether OXA-23-like, OXA-40-like, OXA-58-like or OXA-51-like genes or the ISAba1 insertion sequence were detected
Referral link Form

Details

Demonstration of the presence of an OXA carbapenemase gene by PCR.

Carbapenemase-mediated carbapenem resistance in Acinetobacter is most often due to the production of an OXA-23-like, OXA-40-like or OXA-58-like carbapenemase. In addition, carbapenem resistance in Acinetobacter may be due to overexpression of an OXA-51-like carbapenemase. OXA-51-like carbapenemases are intrinsic in A. baumannii and normally expressed at low levels but they can be overexpressed following the insertion of an ISAba1 sequence element upstream of the OXA-51 gene.

To check for the presence of OXA-23-like, OXA-40-like, OXA-58-like and OXA-51-like genes, a multiplex PCR is used and contains primers for each of the four groups of OXA-type carbapenemases. Any Acinetobacter isolates that are carbapenem resistant, but only positive for OXA-51-like genes in the multiplex PCR, are tested by PCR for the ISAba1 insertion sequence.

This test is appropriate for carbapenem-resistant Acinetobacter or Pseudomonas isolates.