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KPC carbapenemase gene PCR

Registry Details

Area Health Science
Lab name Antibiotic Reference Laboratory
Lab location Kenepuru Science Centre, Porirua
Test name KPC carbapenemase gene PCR
Organisms Enterobacteriaceae
Requested for surveillance yes
Notice required no
Routine turnaround time 6 working days for reference specimens
Culture requirements Pure culture
Media requirements A slope of any medium that supports the growth of the organism
Pre-transport requirements Ambient temperature
Transport requirements Ambient temperature
Unacceptable sample types Clinical specimens will not be accepted.
How results reported The results will state whether the KPC gene was detected
Referral link Form

Details

Demonstration of the presence of a KPC carbapenemase gene by PCR.

KPC carbapenemases are most commonly identified in Klebsiella pneumoniae, but have also been identified in other Enterobacteriaceae. The KPC β-lactamase confers resistance to all β-lactams, including cephalosporins, monobactams and carbapenems. Several types of KPC carbapenemase have been described.

This test is appropriate for isolates that screen positive for carbapenemase in the CLSI modified carbapenem inactivation method.