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IMP, VIM and NDM carbapenemase gene PCR

Registry Details

Area Health Science
Lab name Antibiotic Reference Laboratory
Lab location Kenepuru Science Centre, Porirua
Test name IMP, VIM and NDM carbapenemase gene PCR
Organisms Enterobacteriaceae, Pseudomonas and Acinetobacter
Requested for surveillance yes
Notice required no
Routine turnaround time 6 working days for reference specimens
Culture requirements Pure culture
Media requirements A slope of any medium that supports the growth of the organism
Pre-transport requirements Ambient temperature
Transport requirements Ambient temperature
Unacceptable sample types Clinical specimens will not be accepted.
How results reported The results will state whether IMP, VIM or NDM genes were detected
Referral link Form

Details

Demonstration of the presence of IMP, VIM or NDM metallo-β-lactamase genes by PCR. The test is appropriate for ceftazidime-resistant Enterobacteriaceae, Pseudomonas and Acinetobacter with carbapenem resistance or reduced susceptibility.

Prior to performing the PCR, ESR will screen the isolate for metallo-β-lactamase using the CLSI modified carbapenem inactivation method, and EDTA and MPA/TE inhibitor-based double-disc synergy tests.