Vibrio cholerae
Registry Details
| Area | Health Science |
| Lab name | Enteric Reference Laboratory |
| Lab location | NCBID Wallaceville |
| Test name | Vibrio cholerae |
| Organisms | V. cholerae |
| Requested for surveillance | yes |
| Notice required | yes |
| Routine turnaround time | 10 days |
| Culture requirements | Pure culture |
| Media requirements | Nutrient agar slope |
| Pre-transport requirements | Ambient temperature |
| Transport requirements | Ambient temperature |
| Unacceptable sample types | Mixed cultures will not be tested |
| How results reported | V. cholerae is reported as serotype O1, serotype O139 or non O1 non O139. Serotype O1 organisms are reported as either biotype Classical or biotype El Tor. Biotype El Tor organisms are further subtyped as Ogawa or Inaba. PCR results are reported as positive or negative for cholera toxin and O1/O139 serotype. |
| Referral link | Form |
Description
Identification/confirmation of a Vibrio as V. cholerae consists of serotyping, biochemical tests PCR for detection of toxin. PCR detects the toxin gene ctxA as well as the rfb gene sequence specific for the O1 and O139 serotypes.