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Shigella serotyping and biotyping

Registry Details

Area Health Science
Lab name Enteric Reference Laboratory
Lab location NCBID Wallaceville
Test name Shigella serotyping and biotyping
Organisms Shigella species
Requested for surveillance yes
Notice required no
Routine turnaround time 10 days
Culture requirements Pure culture
Media requirements Nutrient agar slope
Pre-transport requirements Ambient temperature
Transport requirements Ambient temperature
Unacceptable sample types Mixed cultures will not be tested
How results reported The Shigella species and serotype identified is reported. For isolates of Shigella sonnei, a biotype is also reported based on biochemical reactions.Where PCR has been carried out the report will indicate the presence or absence of the vir A and ipai
Referral link Form

Description

The confirmation that the isolate is a Shigella species is done using a collection of biochemical tests in conjunction with serology. Serotyping is done using commercially available antisera. Where an organism cannot be confirmed as Shigella by biochemical and serological means a PCR is performed to detect the presence of the virA and ipaH  virulence genes. These genes are associated with invasiveness in Shigella and also in enteroinvasive Escherichia coli.